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NNK walk strategy

Russian translation: NNK-мутагенез/мутагенез с использованием NNK-кодонов

09:39 Jan 13, 2021
English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: NNK walk strategy
To improve binding to the antigen, an NNK walk strategy on a few residues in the CDR region was used.
NNK = codon


Спасибо!
Alex2013
Russian Federation
Russian translation:NNK-мутагенез/мутагенез с использованием NNK-кодонов
Explanation:
см. напр. использование аналогичных подходов для оптимизации антител:

Так как одновременная оптимизация всех остатков CDR невозможна из-за ограничений размера тестируемых библиотек, для решения этой проблемы используют три подхода: последовательную («CDR walking») модификацию остатков CDR [106], параллельную модификацию остатков CDR и рандомизацию некоторых CDR-петель.
https://www.fbras.ru/wp-content/uploads/2017/10/Altschuler.p...

In saturation mutagenesis, one or more positions along the protein sequence are identified as likely to accommodate beneficial mutations and are then randomized, i.e., the amino acids at these positions are replaced by random ones. The randomization originates at the DNA level, typically via degenerate primers containing a mixture of sequences at the chosen codons. To decrease the chances of introducing a premature stop codon, reduced codon sets are often used: NNB, NNS, and NNK codons (where N = A/C/G/T, B = C/G/T, S = C/G, and K = G/T) are popular choices that still encode all 20 amino acids, but the use of codon sets encoding fewer amino acids has been also advocated
https://aem.asm.org/content/78/1/258
Selected response from:

Igor Andreev
Local time: 05:34
Grading comment
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Summary of answers provided
3NNK-мутагенез/мутагенез с использованием NNK-кодонов
Igor Andreev


  

Answers


4 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
nnk walk strategy
NNK-мутагенез/мутагенез с использованием NNK-кодонов


Explanation:
см. напр. использование аналогичных подходов для оптимизации антител:

Так как одновременная оптимизация всех остатков CDR невозможна из-за ограничений размера тестируемых библиотек, для решения этой проблемы используют три подхода: последовательную («CDR walking») модификацию остатков CDR [106], параллельную модификацию остатков CDR и рандомизацию некоторых CDR-петель.
https://www.fbras.ru/wp-content/uploads/2017/10/Altschuler.p...

In saturation mutagenesis, one or more positions along the protein sequence are identified as likely to accommodate beneficial mutations and are then randomized, i.e., the amino acids at these positions are replaced by random ones. The randomization originates at the DNA level, typically via degenerate primers containing a mixture of sequences at the chosen codons. To decrease the chances of introducing a premature stop codon, reduced codon sets are often used: NNB, NNS, and NNK codons (where N = A/C/G/T, B = C/G/T, S = C/G, and K = G/T) are popular choices that still encode all 20 amino acids, but the use of codon sets encoding fewer amino acids has been also advocated
https://aem.asm.org/content/78/1/258

Igor Andreev
Local time: 05:34
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1427
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