Nov 3, 2011 17:47
12 yrs ago
1 viewer *
English term
hold
English
Medical
Medical: Instruments
influenza assay
From an IFU for an assay for the qualitative detection and identification of influenza A and influenza B viral nucleic acids extracted from nasal swab, nasopharyngeal swab, and nasal aspirate/wash specimens. I am unsure of the meaning of "hold" below. Can somebody please help me out?
”Set the RT-‐PCR cycling times and temperatures at the bottom of the screen as follows:
1. **Hold**
2. Temp: 55.0
3. Secs: 300
4. Optics: OFF”
”Set the RT-‐PCR cycling times and temperatures at the bottom of the screen as follows:
1. **Hold**
2. Temp: 55.0
3. Secs: 300
4. Optics: OFF”
Responses
4 +3 | hold | Sven Petersson |
Responses
+3
39 mins
Selected
hold
konstanthållen temperatur; i motsats till cyklad temperatur
Peer comment(s):
agree |
jccantrell
: Yep, keep the temp constant for 300s instead of moving up or down.
19 mins
|
Thank you very much!
|
|
agree |
Sabine Akabayov, PhD
1 hr
|
Thank you very much!
|
|
agree |
Phong Le
19 hrs
|
Thank you very much!
|
4 KudoZ points awarded for this answer.
Comment: "Stort tack, Sven!"
Discussion
"Make the following changes to the default Thermal Cycler Protocol:
1. Stage 1
a. Reps: 1
b. Temp: 55
c. Time: 5:00
2. Select the bar between Stage 1 and Stage 2. Select the Add **Hold** button to add another stage.