Nov 3, 2011 17:47
12 yrs ago
1 viewer *
English term

hold

English Medical Medical: Instruments influenza assay
From an IFU for an assay for the qualitative detection and identification of influenza A and influenza B viral nucleic acids extracted from nasal swab, nasopharyngeal swab, and nasal aspirate/wash specimens. I am unsure of the meaning of "hold" below. Can somebody please help me out?

”Set the RT-­‐PCR cycling times and temperatures at the bottom of the screen as follows:
1. **Hold**
2. Temp: 55.0
3. Secs: 300
4. Optics: OFF”
Responses
4 +3 hold

Discussion

Åsa_Maria K (asker) Nov 3, 2011:
Another part of the text also mentions "hold":
"Make the following changes to the default Thermal Cycler Protocol:
1. Stage 1
a. Reps: 1
b. Temp: 55
c. Time: 5:00

2. Select the bar between Stage 1 and Stage 2. Select the Add **Hold** button to add another stage.

Responses

+3
39 mins
Selected

hold

konstanthållen temperatur; i motsats till cyklad temperatur

Peer comment(s):

agree jccantrell : Yep, keep the temp constant for 300s instead of moving up or down.
19 mins
Thank you very much!
agree Sabine Akabayov, PhD
1 hr
Thank you very much!
agree Phong Le
19 hrs
Thank you very much!
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4 KudoZ points awarded for this answer. Comment: "Stort tack, Sven!"
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