3' and 5' piggyBac

19:20 Jul 18, 2022
This question was closed without grading. Reason: No acceptable answer

English to Serbian translations [PRO]
Medical - Medical: Pharmaceuticals / tumor antibodies
English term or phrase: 3' and 5' piggyBac
The full human OX40L sequences were codon optimized (Seq ID No: 173) for mammalian expression and cloned into an expression vector under the CMV promoter flanked by 3' and 5' piggyBac specific terminal repeat sequences facilitating stable integration into the cell genome
Svetomir Mijovic
Serbia
Local time: 14:21


Summary of answers provided
3ponovljeni nizovi/repetitivne sekvence, karakteristične za PB transpozon, na 3'-kraju i 5'-kraju
Milan Nesic
Summary of reference entries provided
José Patrício
see
liz askew

  

Answers


2 days 16 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
3' and 5' piggyBac specific terminal repeat sequences
ponovljeni nizovi/repetitivne sekvence, karakteristične za PB transpozon, na 3'-kraju i 5'-kraju


Explanation:
3' and 5' (piggyBac-specific) terminal repeat sequences

"3' and 5'" i "piggyBac-specific" se, zapravo, odnose na "terminal repeat sequences".

"This is usually done by removal of all viral genes between the 5′ and 3′ terminal repeat (TR) sequences and replacing them with the desired transgene(s) (Dong et al., 1996)."

https://www.sciencedirect.com/topics/agricultural-and-biolog...


"An engineered piggyBac transposon with minimal length 5′ and 3′ terminal repeats exhibited greater transposition activity in transfected cultured human cells than a well-characterized hyperactive SB system."

https://www.sciencedirect.com/science/article/pii/S152500161...




    https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/terminal-repeat-sequences
    https://www.sciencedirect.com/science/article/pii/S1525001616312606
Milan Nesic
Native speaker of: Native in SerbianSerbian
PRO pts in category: 120
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Reference comments


1 hr
Reference

Reference information:
piggyBac - no translation

piggyBac 3'- and ''-TRD sequences3
TDR - Transcriptional Repressor Domain (TRD)
' - minute
...
What is a piggyBac vector?
The piggyBac (PB) transposon is a movable genetic element that efficiently transposes between vectors and chromosomes through a “cut-and-paste” - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758974/
piggyback adverb - up on the back and shoulders
The cis-activating function of the 3'-TRD internal domain sequence - https://www.researchgate.net/publication/6568656_Constructio...
The mobility and transposition functions of piggyBac have been established and exploited to develop an important binary system for insect germline transformation [3]. This system consists of a DNA vector, which can be mobilized due to the presence of the piggyBac 5'- and 3'-TRD sequences, and a helper plasmid, which encodes the transposase. The vector also includes a promoter, which controls transcription of an inserted, heterologous gene of interest, and a whole-body or eye color marker, which can be used to identify transgenic offspring. - https://bmcbiotechnol.biomedcentral.com/articles/10.1186/147...

José Patrício
Portugal
Native speaker of: Native in PortuguesePortuguese
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17 hrs
Reference: see

Reference information:
PIGGYBAC Trademark of Poseida Therapeutics, Inc.
https://trademarks.justia.com › piggybac-87939838
PIGGYBAC is a trademark of Poseida Therapeutics, Inc.. Filed in May 29 (2018), the PIGGYBAC covers Reagents, nucleic acids, and nucleotides for scientific ...



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Note added at 17 hrs (2022-07-19 12:49:22 GMT)
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only finding


Систем транспозона ПиггиБац - Википедиа - PiggyBac ...
https://wikisrsr.top › wiki › PiggyBac...
· Translate this page
Систем транспозона ПиггиБац - Википедиа - PiggyBac transposon system ... Тхе ПиггиБац (ПБ) транспосон је мобилни генетски елемент који ефикасно транспонује између ...

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Note added at 17 hrs (2022-07-19 12:50:09 GMT)
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PGDB5
S Wikipedije, slobodne enciklopedije
Idi na navigaciju
Idi na pretragu
PGDB5
Identifikatori
Aliasi
Vanjski ID-jevi GeneCards: [1]
Ortolozi
Vrste Čovjek Miš
Entrez

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Ensembl

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UniProt

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a


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RefSeq (mRNK)

n/a


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RefSeq (bjelančevina)

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Lokacija (UCSC) n/a n/a
PubMed pretraga n/a n/a
Wikipodaci
Pogledaj/uredi – čovjek
PiggyBac izvedeni prenosivi element 5/Transposaza IS4
Identifikatori
Simbol DDE_Tnp_1_7, PGBD
Pfam PF13843
InterPro IPR029526
Dostupne proteinske strukture:
https://bs.wikipedia.org/wiki/PGDB5
PiggyBac izvedeni prenosivi element 5 jest enzim koji je kod ljudi kodiran genom PGBD5 sa hromosoma 1.[1] PGBD5 is a DNA transposase related to the ancient PiggyBac transposaza prci puta identificurana je kod kupudnog moljca, Trichoplusia ni.[2] Vjeruje se da je gen pripitomljen prije više od 500 miliona godina kod zajedničkog pretka glavonožaca i kičmenjaka.[3]

liz askew
United Kingdom
Works in field
Native speaker of: Native in EnglishEnglish
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